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rabbit anti-5hmec  (Active Motif)


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    Active Motif rabbit anti-5hmec
    Rabbit Anti 5hmec, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-5hmec/pmc05399555-197-39-42?v=Active+Motif
    Average 90 stars, based on 1 article reviews
    rabbit anti-5hmec - by Bioz Stars, 2026-07
    90/100 stars

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    ICSI embryos and cloned embryos non-treated (NT) and treated with 100 μM vitamin C (VitC), 10 μM psammaplin A (PsA) or the combination of both (VitC-PsA) during 16 h were immunostained for 5meC and <t>5hmeC</t> detection. A) Representative images of 5meC and 5hmeC staining. Scale bar = 20 μm. B) Average intensity of 5hmeC/5meC signal ratio (+SEM) relative to ICSI embryos.
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    ICSI embryos and cloned embryos non-treated (NT) and treated with 100 μM vitamin C (VitC), 10 μM psammaplin A (PsA) or the combination of both (VitC-PsA) during 16 h were immunostained for 5meC and 5hmeC detection. A) Representative images of 5meC and 5hmeC staining. Scale bar = 20 μm. B) Average intensity of 5hmeC/5meC signal ratio (+SEM) relative to ICSI embryos.

    Journal: PLoS ONE

    Article Title: Improved Development of Somatic Cell Cloned Mouse Embryos by Vitamin C and Latrunculin A

    doi: 10.1371/journal.pone.0120033

    Figure Lengend Snippet: ICSI embryos and cloned embryos non-treated (NT) and treated with 100 μM vitamin C (VitC), 10 μM psammaplin A (PsA) or the combination of both (VitC-PsA) during 16 h were immunostained for 5meC and 5hmeC detection. A) Representative images of 5meC and 5hmeC staining. Scale bar = 20 μm. B) Average intensity of 5hmeC/5meC signal ratio (+SEM) relative to ICSI embryos.

    Article Snippet: The immunofluorescence detection of 5-hydroxymethylcytosine (5hmeC) was also performed, by incubating previously fixed, permeabilized, blocked and 4N HCl-treated embryos first with a 1:500 dilution of rabbit polyclonal anti-5hmeC primary antibody (Active motif, La Hulpe, Belgium) overnight at 4°C, and then with 6 μg/mL goat anti-rabbit Alexa Fluor 594 secondary antibody (Molecular probes, Life technologies, Alcobendas, Spain) for 1h at room temperature.

    Techniques: Clone Assay, Staining